Journal: Frontiers in Microbiology
Article Title: Bacterial metabolites influence the autofluorescence of Clostridioides difficile
doi: 10.3389/fmicb.2024.1459795
Figure Lengend Snippet: Clostridioides difficile autofluorescence is affected by certain sugars and amino acids. C. difficile was grown for 6 h in BHI anaerobically, washed with PBS and then added to Biolog © Phenotypic Microarray plates in PBS at an OD 600nm = 1. Baseline line fluorescence and fluorescence with C. difficile was measured in the green channel (485 nm/528 nm). Compounds are grouped into (A) monosaccharides, (B) disaccharides, (C) trisaccharides, (D) polysaccharides, (E) alcohol sugars, (F) amino sugars, and (G) amino acids.
Article Snippet: Biolog© Phenotypic Microarray plates PM1, PM2a, PM9, and PM10 were used to measure C. difficile autofluorescence with various compounds.
Techniques: Microarray, Fluorescence